*Stem cells therapy
Stem cells have the remarkable potential to develop into many different cell types in the body. In addition, in many tissues they serve as a sort of internal repair system, dividing essentially without limit to replenish other cells.. When a stem cell divides, each new cell has the potential either to remain a stem cell or become another type of cell with a more specialized function, such as a muscle cell, a red blood cell, or a brain cell.

Stem cells are distinguished from other cell types by two important characteristics. First, they are unspecialized cells capable of renewing themselves through cell division, sometimes after long periods of inactivity. Second, under certain physiologic or experimental conditions, they can be induced to become tissue- or organ-specific cells with special functions. In some organs, such as the gut and bone marrow, stem cells regularly divide to repair and replace worn out or damaged tissues. In other organs, however, such as the pancreas and the heart, stem cells only divide under special conditions.

Given their unique regenerative abilities, stem cells offer new potentials for treating diseases such as diabetes, Alzheimer, Parkinson disease, ALS, spine crash and heart disease.
Until recently, scientists primarily worked with two kinds of stem cells from animals and humans:;embryonic stem cells and non-embryonic "somatic" or "adult" stem cells..
Embryonic stem cells can be grown relatively easily in culture. Adult stem cells are rare in mature tissues, so isolating these cells from an adult tissue is challenging, and methods to expand their numbers in cell culture need special know-how.. This is an important distinction, as large numbers of cells are needed for stem cell replacement therapies.

*Our Stem Cells Therapy
We started by using fetal SCs and umbilical blood SCs. Fetal SCs extracted from brain and other parts of nervous system of fetus demonstrated extremely high efficacy in the treatment of parkinsonism, Alzheimerfs disease, stroke, multiple sclerosis, head trauma, other degenerative, traumatic and autoimmune diseases of nervous system. But taking into account moral aspects of  fetal SC using we began to look for more acceptable (from moral point of view) sources of SC.

We came to conclusion that two other sources are suitable and started develop two new technologies:
(1) Cultivation of SCs from female egg discarded during extra corporal fertilization.
(2) Separation and multiplication of SCs separated from fat tissue.

The use of SCs from fat tissue and from female egg demonstrated high efficacy in the same diseases as fetal SCs. The use of SCs from fat tissue and from female egg also demonstrated high potential in re-juvenation therapy. Since then the re-juvenation by means of  SC became in our arsenal of medical services to our patients.

The following has been a list of steps in successful cell-based treatments that we will have to learn to control to bring such treatments to the clinic. To be useful for transplant purposes, stem cells must be reproducibly made to: All of these requirements we cleared in our clinic.

PjProliferate extensively and generate sufficient quantities of tissue under highly clean environment and equipments.

2)Differentiate into the desired cell type(s).

3)Survive in the recipient after transplant.

4)Integrate into the surrounding tissue after transplant.

5)Function appropriately for the duration of the recipient's life.

6)?Avoid harming the recipient in any way.

*Diseases Treated With Stem Cells
Alzheimer's, Autism ,Autoimmune diseases ,Anemias ,Bone and cartilage deformities ,Burns Cancer (brain, breast, ovarian, etc.), Cerebral palsy,Corneal scaring ,Crone's disease ,Diabetes Heart disease,Immunodeficiencies ,Repair of cardiac tissue after MI ,Leukemias ,Lymphomas Melanoma ,Multiple sclerosis ,Multiple myelomas ,Muscular dystrophy ,Neural blastoma Osteoarthritis, Paralysis,Parkinson's Disease ,Rheumatoid arthritis ,Scleroderma Scleromixadema ,Spinal chord damage ,Stroke ,Systemic lupus ,Tendonitis ,ED, Loss of eyesight, ALS

*Q & A
There are several questions and answers which were asked about the use of eggs for SCs in our clinic.

Q-1.  How many stem cells will be used for the treatment of one patient.

A-!.   Although it depends on the purpose of treatment and kinds of diseases, usually 50M to 100M stem cells in total will be injected to a patient at several times for one course of treatment which will take 10 days to 14 days.

Q-2.  How those stem cells are injected to patients.

A-2.  For the repair of brain nerves or nerves in spine etc. such as the case of ALS, Alzheimer's disease, Parkinson's disease, spine injury etc. stem cells will be directly injected into spinal fluid by LP.@However even above cases, subcutaneous or intravenous injection is adopted based on the result of blood examination which showed requirement of less stress for patients. Because LP is very painful so anesthesia is needed. In general cases stem cells will be injected to patients subcutaneously or intravenously by drip. In case of treatment of loss of eye sight by glaucoma, stem cells were injected to the back side of eyeballs.


Q-3. What is difference between fertilized eggs or before fertilization in terms of difference of handling or efficacy as the source of culturing?

A-3 -Unfertilized eggs are more genetically homogenous, they are less overloaded by different kinds of genetic material (there is no material brought by spermatozoa) and theoretically SCs derived from unfertilized eggs should be more flexible regarding transformation to hostfs cells. Flexibility may provide more effectiveness (again theoretically). The less mature is a SC, the more effective it is (the cell in early stage of development is more effective). Unfertilized egg is more earlier stage than fertilized(if we follow the natural course of events).


Q-4. In case of using donated female eggs, the DNA shall be different from patient's DNA. Do you replace the nucleus of egg with patient's nucleus before culturing or nucleus of eggs will be replaced automatically by patientfs DNA after reaching to the destination? Or new cells with different DNA will coexist with original cells of different DNA?


A-4.  I do not replace the DNA of donated eggs with patientfs DNA. First, it is quite complicated and expensive procedure and, second, there is no much sense in that. As you know, transplanted organs, for example, kidney, work having DNA absolutely no identical with hostfs  DNA. They work without transformation of their DNA to hostfs DNA, their problem is immunological rejection. SCs are much more flexible and adaptive to hostfs organism, than the cells of transplanted organs. Sure, it would be excellent to replace the DNA of donated eggs with the DNA of patients, but at present it is too expensive and complicated for being rational in clinical practice.


Q-5.  A person told me he saw a movie on TV about SCs. He told me the movie
showed the first SCs was conducted using female eggs replacing nucleus by patients' nucleus and then cultured. Is this correct or some mistake?

A-5. I suppose that he saw a TV movie about cloning. If you want to have a copy of an organism, you should take an egg and replace its nucleus with the nucleus of SOMATIC CELL THAT CONTAIS DIPLOID SET OF GENES. But you can not have the complete copy of chosen organism, because the new organism (the copy) will have the genes from the nucleus of chosen organism + the genes of cytoplasm of the egg (mitochondria and some other components of cytoplasm have their own genetic material that are independent upon the genes of the nucleus. You should realize that nucleus is not the only source of transmission of genetic data).


*Clinical cases

Here are several case reports which were successfully applied SC therapy at our clinic. You are welcomed to ask us any questions about availability of SC therapy about troubles you want to improve conditions or complete cure.

Patient D., 63 years old, after a year of stroke had the painful shoulder (the latter is one of the most disturbing complications in patients with a residual hemiparesis). Prolonged pressure on a paralyzed limb led to his peripheral nerve lesion that was not recognized earlier because it was superimposed on brain damage resulting from the stroke. As a result the patient had severe shoulder pain (caused by increased traction on the shoulder capsule secondary to abnormal positioning of the paralyzed arm) and significant weakness of his arm determined by brain damage and secondary lesion of paralyzed  limb nerve. All attempts of relieving the pain and restoring the hand function (physical therapy, analgesics, the normal alignment of the joint , use of slind, heat application, peripheral nerve electro stimulation,  medications that facilitate nerve electrical impulse transfer through synapse, etc.) were without results. After starting SC therapy during significant positive results were achieved during  two months: the weakness of the hand was significantly reduced, the patient was able to keep his hand at night in the position that did not cause traction of the shoulder capsule and the pain went away.

Patient M, 58 years old, after 6 years of severe uncontrolled diabetes mellitus had fast progressing retinal detachment and vitreal hemorrhages in both eyes with considerable visual loss. All standard treatments were ineffective and his vision was getting worse and worse. After 3 months he lost his vision completely. Slit lamp examination showed that the treatment dramatically deceased blood in corpus vitreum with restoring of its transparency (both eyes), but it was unable to improve the condition of the retina ? the latter was totally destroyed in both eyes. Concentrated  SCs (3 ml every injection, 5M in one ml, 15M for whole injection, 30 M (15M x 2) in both eyes for every procedure)  were injected retro bulbar once a week ( three times during three week). After 10 days of the beginning of SC treatment the patient started to see and distinguish hand fingers in front of his eyes, slit lamp examination revealed some regeneration of retina and fovea lutea. The patient ? an Armenian who lives in Russia, went home after compleating the procedures and after two months of the last procedure (both eyes injections) sent a telegram that his vision was restored in the limits that  gives him an opportunity to see enough for elementary serving himself.

A case of ALS
A police man has been suffering from ALS for 7 yeas. He is a former police colonel. 4 years ago he went to Germany for treatment and he had all possible treatments including steroids and interferons, etc. He went back in very bad condition, he was not able to breath spontaneously and a special device for artificial ventillation of his lungs (the cost of the device was $18000) was providing his breathing. He was not able to move his hands and legs, he was not able to speak and he had problems with swallowing water and food. All specialists told his relatives that he would be able to live no more than 1-2 years. I started SC therapy and was injecting SCs into his spinal fluid. Very soon he started moving his extremities, swallow water and food and  saying some words. The side effect of SC therapy was headache and pain in the site of spinal punction. The patient is still alive and everybody thinks that it is a miracle. Now his condition is much better than when we started the therapy with SCs, although he is not able to walk yet and continue to have severe weakness in his arms and legs. Because of the side effects (headache and pain in the vertevral column), his relatives stopped SC therapy after 5 injections of SCs. I have decided not to insist on continuation of SC therapy taking into account that the patient is a former policeman and SC therapy is not accepted legally in Armenia yet (you know, policemen are people with some professional trends to ask too much questions). Anyway, SC therapy in this concrete case has demonstrated an excellent result.


 





Dr.Minasyan



Selection of stem cells




Stem cells therapy was confirmed
effective for Alzheimer,Parkinsons,
ALS and many incurable diseases by
conventional medications
 






Process of selecting stem cells
from fat cells and culturing



By injecting stem cells to the
back side of eye balls of a patient
who lost eye sight by grucoma
eye sight was recovered



Picture shows how to inject
stem cells to tha back side
of eye balls



Stem cells therapy is also
effective for animals




Pets became important
family members who need
similar medications like humans






You are invited to come to
Armenia to enjoy visits to
bearutiful places and historical
places. Armenia is known
as the first country to have
decited Christianity as the
national relegion in the world.





This charch was built
2000 years ago and still
used.

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